Enzyme Kits
The need for economic, robust, scalable and reliable processes for the synthesis of chiral APIs and intermediates has resulted in Almac’s process chemists tuning their skills at the interface of chemistry and biology, and indeed embracing biocatalysts and biocatalytic processes in organic synthesis. This paradigm shift has resulted in biocatalysis becoming the work-horse of the chemists tool-box for chiral chemistry.
Biocatalysis is the first choice for Almac for any scale-up chemistry involving chirality.
The acceptance of the biocatalyst has evolved due to its numerous applications in chemical synthesis and its ability to accept multi functional moieties.
Almac has developed a range of hydrolases, carbonyl reductases and transaminases to provide access to a broad range of chiral acids, esters, alcohols and amines and now provides access to libraries of enzymes for customer evaluation. Almac offers a simple business model that avoids ‘IP issues’.
Development and manufacturing quantities are available on a transparent and simple £/Kg basis. Screening for customer-requested biotransformations, process optimisation and manufacture of chiral products by biocatalysis are also available.
Almac offers the following kits:
- Hydrolase biocatalysts -
Screening kits have been built for ease of use and contain 45 commercially available hydrolases (45x500 mg), pre-prepared phosphate buffer (2x200 mL) for hydrolysis and a selection of acyl donors for selective acylation of alcohols and amines (vinyl acetate (1x5 mL), vinyl butyrate (1x5 mL), succinic anhydride (1x5 g), ethyl acetate (1x5 mL) and ethyl butyrate (1x5 mL)). There is also a detailed user guide included.
- Carbonyl Reductase (CRED) biocatalysts -
The kit contains 40 diverse CRED biocatalysts (40x50 mg), pre-prepared phosphate buffer (1x200 mL) ready for use, NAD (1x150 mg) and NADP (1x150 mg) cofactors, DMSO (1x5 mL), glucose dehydrogenase (GDH, 1x 500 mg) and glucose (1x5 g) for the recycle system. A detailed user guide is also included.
- Nitrilase and Nitrile Hydratase (NHase) biocatalysts -
Screening kits consist of 9 NHases (9x50 mg) and 5 Nitrilases (5x50 mg), pre-prepared phosphate buffer (1x200 mL) for hydrolysis, DMSO (1x5 mL) and a user guide.
- Aldehyde Reductase (ARED)- The kit contains 16 diverse ARED biocatalysts (16x50 mg), pre-prepared phosphate buffer (1x200 mL) ready for use, NAD (1x80 mg) and NADP (1x80 mg) cofactors, DMSO (10 mL), glucose dehydrogenase (GDH, 1x250 mg) and glucose (1x2.5 g) for the recycle system. A detailed user guide is also included.
- Transaminase (currently under investigation)
R&D quantities and commercial supply of the biocatalysts are available upon request. |
